Detection of abundantly transcribed genes and gene translocation in human immunodeficiency virus-associated non-Hodgkin's lymphoma

Neoplasia. Mar-Apr 2001;3(2):132-42. doi: 10.1038/sj.neo.7900137.

Abstract

Several novel, differentially transcribed genes were identified in one centroblastic and one immunoblastic HIV-associated B-cell non-Hodgkin's lymphoma (B-NHL) by subtractive cloning. In both lymphomas, we detected an upregulated transcription of several mitochondrial genes. In the centroblastic B-NHL, we found a high level transcription of nuclear genes including the interferon-inducible gene (INF-ind), the immunoglobulin light chain gene (IgL), the set oncogene, and several unknown genes. The data obtained on upregulated expression of the genes in human B-NHL of HIV-infected patients considerably overlap with those obtained earlier for the B-NHL of simian immunodeficiency virus-infected monkeys. In the centroblastic lymphoma, one transcript revealed a fusion of the 3'-untranslated region of the set gene and the C-terminal region of the IgL gene. This chimeric sequence was confirmed by a site-directed polymerase chain reaction performed with total cDNA and genomic DNA. The expected amplification product was obtained in both cases pointing to a genomic rearrangement. The IgL-set fusion sequence was not found in cDNA preparations and genomic DNA of the immunoblastic HIV-associated B-NHL. Further studies are necessary to determine whether these genes contribute to lymphoma development or can be used as therapeutic targets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Databases, Factual
  • Dose-Response Relationship, Drug
  • Humans
  • Immunoblotting
  • Immunoglobulins / metabolism
  • Lymphoma / metabolism
  • Lymphoma, AIDS-Related / metabolism*
  • Lymphoma, Non-Hodgkin / virology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism*
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic*
  • Up-Regulation

Substances

  • 3' Untranslated Regions
  • DNA, Complementary
  • Immunoglobulins
  • RNA, Messenger