Evaluation of recomWell Treponema, a novel recombinant antigen-based enzyme-linked immunosorbent assay for the diagnosis of syphilis

Clin Microbiol Infect. 2001 Apr;7(4):200-5. doi: 10.1046/j.1469-0691.2001.00232.x.


Objective: To evaluate the diagnostic performance of an enzyme immunosorbent assay (recomWell Treponema) for the diagnosis of syphilis. The novel recombinant antigens Tpn47, TpN17 and TpN15 were utilized.

Methods: A total of 782 human serum specimens, belonging to four different categories (blood donors, n = 200; routine laboratory screening for syphilis, n = 400; syphilis patients, n = 122; potential cross-reactors, n = 60), were evaluated to compare the sensitivity and specificity of the recomWell Treponema kit with a standard whole Treponema pallidum cell lysate antigen-based ELISA (Syphilis Screening) and with micro-haemagglutination (MHA-TP).

Results: The overall specificity and sensitivity of the recomWell Treponema IgG was 98.9% and 98.3%, respectively. The specificity and sensitivity of Syphilis Screening ELISA was 98.7% and 98.3%, respectively. The agreement between recomWell Treponema and Syphilis Screening was 100%, 97.8%, 95.9% and 95% among the blood donor specimens, screening samples, syphilis specimens and the potential cross-reactors, respectively. Values of concordance varying from 96.7% to 98.3% were found in the different groups of sera between recomWell Treponema and MHA-TP. In addition, recomWell Treponema demonstrated a good diagnostic performance when used to detect the IgM to T. pallidum. No false-positive sera were identified and, in 17/19 samples from primary infection, an IgM immune response was found.

Conclusions: recomWell Treponema was shown to be a highly specific and sensitive method in all stages of syphilis screening and it can be considered as alternative to other ELISA tests based on native antigen preparations.

Publication types

  • Evaluation Study

MeSH terms

  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / immunology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / standards
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Reagent Kits, Diagnostic
  • Recombinant Proteins / immunology
  • Sensitivity and Specificity
  • Syphilis / blood
  • Syphilis / diagnosis*
  • Syphilis Serodiagnosis / methods*
  • Syphilis Serodiagnosis / standards
  • Treponema pallidum / immunology*


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Immunoglobulin G
  • Immunoglobulin M
  • Reagent Kits, Diagnostic
  • Recombinant Proteins