Hydrogen excretion upon ingestion of dairy products in lactose-intolerant male subjects: importance of the live flora

Eur J Clin Nutr. 2001 Jun;55(6):509-12. doi: 10.1038/sj.ejcn.1601169.


Objective: To assess the effects of the ingestion of milk, yoghurt (10(8) bacteria/ml), heat-treated yoghurt (<15 bacteria/ml) and two products obtained by dilution of yoghurt with heat-treated product (10(6) and 10(5) bacteria/ml) on hydrogen production and symptoms of lactose intolerance in lactose malabsorbers.

Design: Double-blind, randomised cross-over design.

Setting: The study was performed in the phase 1 clinical unit of OPTIMED, Nancy, France.

Subjects: Twenty-four male lactose malabsorbers were selected for the study.

Interventions: Hydrogen production and adverse events were followed during 8 h after ingestion of the products.

Results: The results clearly demonstrate that ingestion of yoghurt with 10(8) bacteria/ml leads to lower H(2) excretion and complaints than the other products. Results observed with the products containing a reduced population of live flora remain better than those observed with milk.

Conclusions: The importance of a high population of the live flora is underlined.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Breath Tests
  • Colony Count, Microbial
  • Cross-Over Studies
  • Dairy Products / microbiology*
  • Double-Blind Method
  • Humans
  • Hydrogen / metabolism*
  • Lactose / metabolism*
  • Lactose Intolerance / physiopathology*
  • Male
  • Milk / microbiology
  • Temperature
  • Time Factors
  • Yogurt / microbiology


  • Hydrogen
  • Lactose