T cells engrafted with a recombinant anti-CD30 receptor target autologous CD30(+) cutaneous lymphoma cells

Gene Ther. 2001 Jun;8(11):891-5. doi: 10.1038/sj.gt.3301467.


T cells can be directed to antigen-specific, MHC-independent target cell lysis by grafting with a recombinant receptor with antibody-like specificity. Here, we asked whether T cells from the peripheral blood of a patient with cutaneous T cell lymphoma can be recruited for an immune response against autologous tumor cells. Lymphoma cells with a CD3(-) CD4(+) CD30(+) phenotype and clonal TCR-Vbeta7 rearrangement were isolated from a cutaneous lesion. The lymphoma lesion additionally harbored CD3(+) CD25(+) activated normal T cells despite ongoing tumor progression. Peripheral blood-derived T cells from the lymphoma patient were retrovirally engrafted with a recombinant anti-CD30-scFv-gamma receptor. Upon cocultivation with autologous CD30(+)lymphoma cells, grafted T cells increase IFN-gamma secretion and lyse specifically lymphoma cells with high efficiency, even at an effector to target cell ratio of as low as 1:20. Our data demonstrate that the recombinant anti-CD30-gamma receptor overcomes T cell tolerance for tumor cells and directs T cells specifically against autologous lymphoma cells.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cell Separation
  • Coculture Techniques
  • Flow Cytometry
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Genetic Therapy / methods*
  • Genetic Vectors / administration & dosage
  • Humans
  • Immunotherapy, Adoptive / methods*
  • Ki-1 Antigen*
  • Lymphocyte Activation
  • Lymphoma, T-Cell, Cutaneous / immunology
  • Lymphoma, T-Cell, Cutaneous / therapy*
  • Male
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Recombinant Proteins / genetics
  • Retroviridae / genetics
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • Transduction, Genetic


  • Ki-1 Antigen
  • Receptors, Antigen, T-Cell, alpha-beta
  • Recombinant Proteins