Protection of rainbow trout against infectious hematopoietic necrosis virus four days after specific or semi-specific DNA vaccination

Vaccine. 2001 Jul 16;19(28-29):4011-9. doi: 10.1016/s0264-410x(01)00113-x.


A DNA vaccine against a fish rhabdovirus, infectious hematopoietic necrosis virus (IHNV), was shown to provide significant protection as soon as 4 d after intramuscular vaccination in 2 g rainbow trout (Oncorhynchus mykiss) held at 15 degrees C. Nearly complete protection was also observed at later time points (7, 14, and 28 d) using a standardized waterborne challenge model. In a test of the specificity of this early protection, immunization of rainbow trout with a DNA vaccine against another fish rhabdovirus, viral hemorrhagic septicemia virus, provided a significant level of cross-protection against IHNV challenge for a transient period of time, whereas a rabies virus DNA vaccine was not protective. This indication of distinct early and late protective mechanisms was not dependent on DNA vaccine doses from 0.1 to 2.5 microg.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cross Reactions
  • Dose-Response Relationship, Immunologic
  • Fish Diseases / immunology*
  • Fish Diseases / prevention & control*
  • Injections, Intramuscular
  • Oncorhynchus mykiss / immunology*
  • Oncorhynchus mykiss / virology*
  • Rhabdoviridae / genetics*
  • Rhabdoviridae / immunology*
  • Rhabdoviridae Infections / immunology
  • Rhabdoviridae Infections / prevention & control
  • Rhabdoviridae Infections / veterinary*
  • Time Factors
  • Vaccines, DNA / administration & dosage*
  • Vaccines, DNA / genetics
  • Viral Vaccines / administration & dosage*
  • Viral Vaccines / genetics


  • Vaccines, DNA
  • Viral Vaccines