Sequence analysis of zebrafish chondromodulin-1 and expression profile in the notochord and chondrogenic regions during cartilage morphogenesis

Mech Dev. 2001 Jul;105(1-2):157-62. doi: 10.1016/s0925-4773(01)00417-8.


Chondromodulin-I (ChM-I) is suggested in higher vertebrate systems to function as a key regulatory protein for cartilage development. To further understand the process of chondrogenesis and the function of ChM-I, we have cloned the zebrafish cDNA for chondromodulin-1 (chm1) and have mapped the chm1 gene locus. The expression profile of chm1 was determined during zebrafish embryonic development and compared to that of type II collagen (col2a1). Maternal chm1 transcripts were detected before midblastula transition and zygotic expression of chm1 was first observed in the notochord at the 10-somite stage. At later developmental stages, chm1 expression was detected in areas surrounding the otic vesicles, in the developing craniofacial cartilage elements, and in the chondrogenic region of the pectoral fins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cartilage / embryology*
  • Cattle
  • DNA, Complementary / metabolism
  • Growth Substances / biosynthesis*
  • Growth Substances / genetics*
  • Humans
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins*
  • Membrane Proteins*
  • Molecular Sequence Data
  • Notochord / metabolism*
  • Phylogeny
  • Radiation Hybrid Mapping
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Time Factors
  • Tissue Distribution
  • Zebrafish
  • Zygote / metabolism


  • DNA, Complementary
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Membrane Proteins
  • CNMD protein, human