Block-replacement mutagenesis for functional dissection of multiple transcription factor complexes

Biomol Eng. 2001 Aug;18(1):9-11. doi: 10.1016/s1389-0344(01)00080-6.


We propose a simple method for investigation of roles of individual transcription factors in complexes of multiple factors bound to the same cis element. By block-replacement mutagenesis, the whole cis element is replaced with a new one containing a binding site for a single factor. From the reporter activity of the mutant promoter construct, the importance of the individual factor in transcriptional activation is deduced. This approach allowed us to functionally identify SP1 as the most important PR1 binding transcription factor in the MN promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression Regulation*
  • Molecular Biology / methods*
  • Mutagenesis, Site-Directed*
  • Regulatory Sequences, Nucleic Acid / genetics*
  • Transcription Factors / metabolism*


  • Transcription Factors