Abstract
The cbbR encoding the LysR-type transcriptional regulator is located downstream of cbbLSQOYA and this gene is located upstream of cbbFPT in divergent transcription. The two promoter regions with LysR-binding sites are located in the cbbL upstream region and in the cbbR-cbbF intergenic region. Electrophoretic mobility shift assays using a cell extract of Escherichia coli harboring a plasmid containing cbbR and the DNA fragments of promoter regions indicated that CbbR binds in both regions. NADPH caused differences in the complex of CbbR and DNA.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / metabolism
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Base Sequence
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Betaproteobacteria / genetics*
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Betaproteobacteria / metabolism*
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Binding Sites
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism*
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Escherichia coli / genetics
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Molecular Sequence Data
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Promoter Regions, Genetic*
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Restriction Mapping
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
Substances
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Bacterial Proteins
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DNA, Bacterial
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DNA-Binding Proteins
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Transcription Factors
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cfxR protein, bacteria
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LysR protein, Bacteria