CbbR, a LysR-type transcriptional regulator from Hydrogenophilus thermoluteolus, binds two cbb promoter regions

FEMS Microbiol Lett. 2001 May 1;198(2):151-7. doi: 10.1111/j.1574-6968.2001.tb10635.x.


The cbbR encoding the LysR-type transcriptional regulator is located downstream of cbbLSQOYA and this gene is located upstream of cbbFPT in divergent transcription. The two promoter regions with LysR-binding sites are located in the cbbL upstream region and in the cbbR-cbbF intergenic region. Electrophoretic mobility shift assays using a cell extract of Escherichia coli harboring a plasmid containing cbbR and the DNA fragments of promoter regions indicated that CbbR binds in both regions. NADPH caused differences in the complex of CbbR and DNA.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Betaproteobacteria / genetics*
  • Betaproteobacteria / metabolism*
  • Binding Sites
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / genetics
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*


  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Transcription Factors
  • cfxR protein, bacteria
  • LysR protein, Bacteria