The MgtE Mg2+ Transport Protein Is Involved in Aeromonas Hydrophila Adherence

FEMS Microbiol Lett. 2001 May 1;198(2):189-95. doi: 10.1111/j.1574-6968.2001.tb10641.x.

Abstract

Aeromonas hydrophila AH-3 strains carrying mutations in mgtE, which encodes a Mg2+ and Co2+ transport system, showed a 50% reduction of in vitro adherence to HEp-2 cells, a reduction in swarming in semisolid swarming agar, and decrease in biofilm formation of over 60% in comparison to the wild-type strain. The cloned A. hydrophila mgtE expressed from a plasmid complements a Salmonella typhimurium strain deleted for all Mg2+ transporters both phenotypically and by measurement of 57Co2+ uptake. Likewise, plasmid-borne mgtE was able to complement the changes observed in A. hydrophila mgtE mutants. We suggest that MgtE and thus Mg2+ and possibly Co2+ have a role in A. hydrophila related to their swarming ability and related consequences such as adherence and biofilm formation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas hydrophila / genetics
  • Aeromonas hydrophila / physiology*
  • Antiporters / genetics
  • Antiporters / metabolism*
  • Bacterial Adhesion / physiology*
  • Bacterial Proteins / metabolism
  • Biofilms
  • Biological Transport
  • Cloning, Molecular
  • Cobalt / metabolism
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Escherichia coli / physiology
  • Genetic Complementation Test
  • Humans
  • Magnesium / metabolism*
  • Movement / physiology
  • Mutation
  • Restriction Mapping
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / physiology
  • Sequence Analysis, DNA
  • Tumor Cells, Cultured

Substances

  • Antiporters
  • Bacterial Proteins
  • DNA, Bacterial
  • MgtE protein, bacteria
  • Cobalt
  • Magnesium