Regulated ARE-mediated mRNA Decay in Saccharomyces Cerevisiae

Mol Cell. 2001 Jun;7(6):1191-200. doi: 10.1016/s1097-2765(01)00279-9.

Abstract

The stability of several oncogene, cytokine, and growth factor transcripts is tightly regulated by signaling pathways through an ARE (AU-rich element) present in their 3'-UTRs. We have identified a yeast transcript, TIF51A, whose stability is regulated through its AU-rich 3'-UTR. We demonstrate that the mammalian TNFalpha and c-fos AREs regulate turnover of a reporter yeast transcript in a similar manner. AREs stabilize the transcript in glucose media and function as destabilizing elements in media lacking glucose or when the Hog1p/p38 MAP kinase pathway is inhibited. Significantly, both yeast and mammalian AREs promote deadenylation-dependent decapping in the yeast system. Furthermore, the yeast ELAV homolog, Pub1p, regulates the stability mediated by the TNFalpha ARE. These results demonstrate that yeast possess a regulatable mechanism for ARE-mediated decay and suggest conservation of this turnover process from yeast to humans.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3' Untranslated Regions / metabolism
  • Adenosine / metabolism
  • Animals
  • Base Sequence
  • Gene Expression Regulation, Fungal*
  • Genes, Reporter
  • In Vitro Techniques
  • Mammals
  • Mitogen-Activated Protein Kinases / metabolism
  • Molecular Sequence Data
  • Peptide Initiation Factors / genetics
  • Poly(A)-Binding Proteins*
  • Polymers / metabolism
  • Proto-Oncogene Proteins c-fos / metabolism
  • RNA Processing, Post-Transcriptional / physiology
  • RNA, Fungal / metabolism*
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism*
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins*
  • Tumor Necrosis Factor-alpha / genetics
  • p38 Mitogen-Activated Protein Kinases

Substances

  • 3' Untranslated Regions
  • PUB1 protein, S cerevisiae
  • Peptide Initiation Factors
  • Poly(A)-Binding Proteins
  • Polymers
  • Proto-Oncogene Proteins c-fos
  • RNA, Fungal
  • RNA, Messenger
  • RNA-Binding Proteins
  • Saccharomyces cerevisiae Proteins
  • Tumor Necrosis Factor-alpha
  • eukaryotic translation initiation factor 5A
  • polyadenosine
  • HOG1 protein, S cerevisiae
  • Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases
  • Adenosine