1. Stimulation of purinergic receptors on retinal pigment epithelial (RPE) cells can increase the rate of fluid transport or decrease phagocytosis. This study aims to: determine whether the purine ATP can be released from RPE cells, begin probing the mechanism of any release and test whether cells degrade ATP extracellularly. 2. ATP release was monitored from cultured human ARPE-19 cells with the luciferin-luciferase assay. Biphasic release of ATP was triggered by basic fibroblast growth factor (bFGF), by the pyrimidine uridine triphosphate (UTP) and by hypotonicity. 3. The Cl(-) channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB) inhibited release of ATP, suggesting that release was associated with Cl(-) channels. 4. Elevating intracellular Ca(2+) directly with ionomycin was insufficient to trigger ATP release. 5. UTP induced a biphasic elevation in intracellular Ca(2+). NPPB inhibited the second phase, suggesting autostimulation by released ATP. 6. Cells grown on permeable supports showed apical release of ATP, analogous to release into subretinal space in vivo. 7. The presence of ecto-ATPases on ARPE-19 cell membranes was suggested by the degradation of ATP added to intact cells. 8. Phagocytosis of fluorescent beads was inhibited by ATP, but the ecto-5'-nucleotidase inhibitor alpha, beta-methylene ADP prevented this, suggesting that inhibition was mediated by extracellular conversion of ATP to adenosine. 9. These results suggest that growth factors, pyrimidines and changes in tonicity could trigger ATP release into subretinal space. The levels of ATP released may be capable of autocrine stimulation of ATP receptors, while conversion to adenosine by ecto-enzymes could alter phagocytosis.