cDNA cloning, chromosomal localization and evolutionary analysis of mouse vacuolar ATPase subunit D, Atp6m

Cytogenet Cell Genet. 2001;92(3-4):337-41. doi: 10.1159/000056924.


The multi-subunit vacuolar ATPase pump uses ATP hydrolysis to move protons into membrane bound compartments. The pump is involved in a variety of cellular functions, including regulation of cytosolic pH, vesicular transport, endocytosis, secretion, and apoptosis. Here, we describe the cDNA cloning and chromosomal mapping of subunit D of murine V-ATPase. The mouse gene, designated Atp6m, maps to Chromosome 12, in a region of high homology with human chromosome 14q24. Evolutionary analysis of subunit D orthologs in a variety of other species reveals that this is a highly conserved protein that has been under remarkably strong negative selection during evolution, most likely reflecting its critical role in multiple cellular processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / chemistry
  • Adenosine Triphosphatases / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 14 / genetics
  • Cloning, Molecular
  • Conserved Sequence / genetics
  • Crosses, Genetic
  • DNA, Complementary / genetics
  • Evolution, Molecular*
  • Female
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mitochondrial Proton-Translocating ATPases
  • Molecular Sequence Data
  • Phylogeny
  • Protein Subunits
  • Sequence Alignment


  • DNA, Complementary
  • MT-ATP6 protein, human
  • Protein Subunits
  • Adenosine Triphosphatases
  • Mitochondrial Proton-Translocating ATPases
  • mt-Atp6 protein, mouse

Associated data

  • GENBANK/AF298810