Bifidobacterium Longum as a Delivery System for Gene Therapy of Chemically Induced Rat Mammary Tumors

Breast Cancer Res Treat. 2001 Mar;66(2):165-70. doi: 10.1023/a:1010644217648.

Abstract

A fundamental obstacle in cancer gene therapy is the specific targeting of therapy directly to a solid tumor, and no systemic delivery system yet exists. A strain of domestic bacteria, Bifidobacterium longum, which is nonpathogenic and anaerobic, selectively localized to and proliferated in 7,12-dimethylbenz[a]anthracene-induced rat mammary tumors after systemic application. We further ascertained the tumor specificity of genetically engineered, as well as wild-type, Bifidobacterium longum. This is the first demonstration that Bifidobacterium longum can be utilized as a specific gene delivery vector for gene therapy on solid breast tumors.

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene
  • Animals
  • Bifidobacterium / genetics*
  • DNA Primers
  • Drug Delivery Systems
  • Female
  • Genetic Engineering
  • Genetic Therapy*
  • Mammary Neoplasms, Experimental / chemically induced
  • Mammary Neoplasms, Experimental / therapy*
  • Polymerase Chain Reaction
  • Rats
  • Rats, Sprague-Dawley

Substances

  • DNA Primers
  • 9,10-Dimethyl-1,2-benzanthracene