Phenotypic changes in Arabidopsis caused by expression of a yeast vacuolar Ca2+/H+ antiporter

Plant Mol Biol. 2001 May;46(1):57-65. doi: 10.1023/a:1010620227913.


In plants, cytosolic Ca2+ levels are tightly regulated, and changes in cytosolic Ca2+ have been implicated in converting numerous signals into adapted responses. Vacuolar ion transporters are thought to be key mediators of cytosolic Ca2+ concentrations. In an attempt to interpret the role of vacuolar Ca2+ transport in plant processes, we have expressed the yeast vacuolar Ca2+/H+ antiporter, VCX1, in Arabidopsis and tobacco. This transporter localizes to the plant vacuolar membrane. VCX1-expressing Arabidopsis plants displayed increased sensitivity to sodium and other ions. These ion sensitivities could be suppressed by addition of calcium to the media. VCX1-expressing plants demonstrated increased tonoplast-enriched Ca2+/H+ antiport activity as well as increased Ca2+ accumulation. These results suggest that VCX1 expression in Arabidopsis could be a valuable tool with which to experimentally dissect the role of Ca2+ transport around the plant vacuole.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antiporters / genetics*
  • Antiporters / metabolism
  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Calcium / metabolism
  • Calcium / pharmacology
  • Calcium-Binding Proteins / genetics*
  • Calcium-Binding Proteins / metabolism
  • Cation Transport Proteins*
  • Fungal Proteins / genetics*
  • Gene Expression
  • Gene Expression Regulation, Plant
  • Phenotype
  • Plants, Genetically Modified / drug effects
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • Plants, Toxic
  • Saccharomyces cerevisiae / genetics
  • Tobacco / genetics


  • Antiporters
  • Calcium-Binding Proteins
  • Cation Transport Proteins
  • Fungal Proteins
  • calcium-hydrogen antiporters
  • Calcium