Analysis of glucosinolates from broccoli and other cruciferous vegetables by hydrophilic interaction liquid chromatography

J Chromatogr A. 2001 Jun 15;919(2):299-304. doi: 10.1016/s0021-9673(01)00842-1.


While methods for the identification and quantification of total plant glucosinolate content typically utilize desulfation of glucosinolates followed by reversed-phase chromatography, the analysis of intact glucosinolates has been problematic. Hydrophilic interaction chromatography offers a novel method for analyzing intact glucosinolates and when performed along with ion-pair reversed-phase chromatography offers a powerful and complementary method for glucosinolate analysis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Glucosinolates / analysis*
  • Vegetables / chemistry*


  • Glucosinolates