Aims: A simple and rapid method was described for DNA isolation directly from activated sludge or other environmental sources, including soil and sediments.
Methods and results: The present method is based on microwave thermal shock and provides DNA suitable for further analysis. It is also effective for RNA extraction.
Conclusion: The protocol is effective, easy, fast and does not require the use of expensive equipment or reagents.
Significance and impact of the study: The described method can be applied to difficult substrates in environmental microbiology studies.