Expression, function, and clinical implications of the estrogen receptor beta in human lung cancers

Biochem Biophys Res Commun. 2001 Jul 13;285(2):340-7. doi: 10.1006/bbrc.2001.5158.


The higher frequency of human lung adenocarcinoma in females than in males, strongly suggests the involvement of gender dependent factors in the etiology of this disease. This is the first investigation of estrogen receptor (ER) beta in human lung. Immunohistochemical staining revealed ERbeta expression in normal lung and in atypical adenomatous hyperplasia (AAH), considered as a precancerous lesion for adenocarcinomas. Adenocarcinomas showed significantly higher expression of ERbeta than squamous cell carcinomas. On the contrary, ERalpha expression was not detected in all cases. The functional integrity of ERbeta such as the binding ability to estrogen responsive element (ERE) and transcriptional activity was confirmed using a human lung cancer cell line, RERF-LC-OK. Colony formation of this cell was significantly reduced in the presence of pure antiestrogen. We conclude that ERbeta, but not ERalpha, is present in lung tissues with an important physiological function in normal lung. Furthermore, ERbeta may play a role in growth and development of adenocarcinomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics
  • Adenocarcinoma / pathology
  • Adenocarcinoma / physiopathology
  • Aged
  • Bronchi / cytology
  • Bronchi / physiology
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / pathology
  • Carcinoma, Squamous Cell / physiopathology
  • Cell Adhesion
  • Estrogen Receptor beta
  • Female
  • Genes, Reporter
  • Humans
  • Hyperplasia
  • Lung / cytology
  • Lung / pathology
  • Lung / physiology
  • Lung Neoplasms / genetics
  • Lung Neoplasms / pathology*
  • Lung Neoplasms / physiopathology
  • Male
  • Middle Aged
  • Receptors, Estrogen / analysis
  • Receptors, Estrogen / genetics
  • Receptors, Estrogen / physiology*
  • Reference Values
  • Respiratory Mucosa / cytology
  • Respiratory Mucosa / physiology
  • Transfection
  • Tumor Cells, Cultured


  • Estrogen Receptor beta
  • Receptors, Estrogen