Change in expression of GBP28/adiponectin in carbon tetrachloride-administrated mouse liver

Biochem Biophys Res Commun. 2001 Jul 13;285(2):372-7. doi: 10.1006/bbrc.2001.5134.


GBP28 (gelatin-binding protein of 28 kDa)/adiponectin is an adipocyte-producing plasma protein proposed to interact with the extracellular matrix. To examine the production of GBP28 in non-adipose tissues, we herein analyzed its expression and localization in mouse livers before and after CCl(4) treatment. In immunohistochemical analyses, the boundary of hepatocytes provided positive signals for GBP28 after 3-6 h and their cytoplasm was intensely stained after 18 h of CCl(4) treatment. Quantitative RT-PCR and in situ hybridization revealed that GBP28 mRNA expression was markedly elevated in CCl(4)-treated mouse livers. These results suggest that the circulating GBP28 binds the extracellular matrices of hepatocytes during the initial stage of CCl(4)-induced hepatic injury and the damaged hepatocytes themselves started to produce GBP28 thereafter. The induced expression of GBP28 was also observed in human hepatoma HepG2 cells after treatment with IL-6. Thus, GBP28 is also produced by the liver, where it undergoes tissue damage-induced transcriptional regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiponectin
  • Animals
  • Antisense Elements (Genetics)
  • Carbon Tetrachloride / toxicity*
  • Carcinoma, Hepatocellular
  • Gene Expression Regulation / drug effects*
  • Humans
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-6 / pharmacology
  • Kinetics
  • Liver / cytology
  • Liver / drug effects
  • Liver / metabolism*
  • Liver Neoplasms
  • Mice
  • Mice, Inbred BALB C
  • Proteins / analysis
  • Proteins / genetics*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic / drug effects
  • Tumor Cells, Cultured


  • Adiponectin
  • Antisense Elements (Genetics)
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-6
  • Proteins
  • RNA, Messenger
  • Carbon Tetrachloride