Insulin Resistance and Type 2 Diabetes Are Not Related to Resistin Expression in Human Fat Cells or Skeletal Muscle

Biochem Biophys Res Commun. 2001 Jul 13;285(2):561-4. doi: 10.1006/bbrc.2001.5173.

Abstract

Resistin is secreted by rodent fat cells and was recently postulated to be an important link between obesity and insulin resistance. We examined Resistin gene expression with real-time RT-PCR in human isolated fat cells, adipose tissue, and muscle from 42 individuals of varying degrees of overweight and who had normal insulin sensitivity or were insulin-resistant or Type 2 diabetic. Resistin was not expressed in human muscle nor was it expressed in most human isolated fat cells or intact biopsies. No difference was found between normal, insulin-resistant, or Type 2 diabetic samples. However, a very low but specific Resistin expression could be demonstrated in isolated fat cells and intact adipose tissue from some individuals (n = 3 and n = 4, respectively). There was no evidence for the expression of splice variants in the human samples. Thus, Resistin does not seem to be an important link to insulin resistance and Type 2 diabetes in human.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism*
  • Alternative Splicing
  • Animals
  • Base Sequence
  • DNA Primers
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / genetics*
  • Genetic Variation
  • Hormones, Ectopic / genetics*
  • Humans
  • Insulin Receptor Substrate Proteins
  • Insulin Resistance / genetics*
  • Intercellular Signaling Peptides and Proteins*
  • Leukocytes / metabolism
  • Mice
  • Middle Aged
  • Muscle, Skeletal / metabolism*
  • Nerve Growth Factor
  • Oligonucleotide Probes
  • Phosphoproteins / genetics
  • Proteins*
  • Resistin
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • DNA Primers
  • Hormones, Ectopic
  • IRS1 protein, human
  • Insulin Receptor Substrate Proteins
  • Intercellular Signaling Peptides and Proteins
  • Irs1 protein, mouse
  • Oligonucleotide Probes
  • Phosphoproteins
  • Proteins
  • RETN protein, human
  • RETNLB protein, human
  • Resistin
  • Retn protein, mouse
  • Retnla protein, mouse
  • Nerve Growth Factor