Beta-galactofuranoside glycoconjugates on conidia and conidiophores of Aspergillus niger

FEMS Microbiol Lett. 2001 Jul 10;201(1):21-7. doi: 10.1111/j.1574-6968.2001.tb10727.x.


Galactose in the furanoic conformation appears to be limited to bacteria and lower eukaryotes. Galactofuranoic (Galf)-containing glycoconjugates that occur in organisms pathogenic or allergenic to man are frequently antigenic and immunodominant. We have used an immunochemical approach, employing a monoclonal antibody that recognises Galf epitopes, to investigate the presence of Galf-containing glycoconjugates within conidia and conidiophores of Aspergillus niger. ELISA and immunofluorescence microscopy indicated that specific and saturable binding sites were found on both. Inhibition studies confirmed that this binding was to Galf-containing glycoconjugates. Interestingly, the conidiophore heads were particularly rich in these glycoconjugates. Western blotting identified a Galf glycoprotein of 150-200 kDa from disrupted conidia.

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Aspergillus niger / chemistry*
  • Aspergillus niger / physiology
  • Carbohydrate Conformation
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes
  • Galactose / analysis*
  • Glycoconjugates / analysis*
  • Glycoconjugates / chemistry
  • Glycoconjugates / immunology
  • Microscopy, Fluorescence
  • Spores, Fungal / chemistry


  • Antibodies, Monoclonal
  • Epitopes
  • Glycoconjugates
  • Galactose