Interaction of diet and training on endurance performance in rats

Exp Physiol. 2001 Jul;86(4):499-508. doi: 10.1113/eph8602158.


We determined the interaction of diet and training on metabolic adaptations in skeletal muscle and liver, and the consequences of these adaptations for endurance. Eighty rats performed a baseline treadmill run to exhaustion at 16 m min(-1) (RUN1) and were then divided into two groups and given one of two diets: high carbohydrate (CHO) or high fat (FAT). Each dietary group was then divided into one of four subgroups: sedentary control that performed no training (NT); low-intensity running (8 m min(-1); LOW) and two groups who trained at their maximal voluntary running speed without electrical stimulation (28 m min(-1); VMAX). Training volume was identical for LOW and VMAX (1000 m session(-1)) and animals ran 4 days week(-1) for 8 weeks. To assess the interaction of the higher intensity exercise with diet, a second endurance test (RUN2) was undertaken after 6 weeks at either 16 m min(-1) or 28 m min(-1). The NT group ran for a longer duration (increase of 77 %) after FAT than CHO (239 +/- 28 vs. 135 +/- 30 min, P < 0.05) at 16 m min(-1). There were no differences in RUN2 for the LOW group when rats ran at 16 m min(-1) (454 +/- 86 vs. 427 +/- 75 min for CHO and FAT groups, respectively), but rats in the VMAX group fed FAT ran longer than rats fed CHO at 28 m min(-1) (100 +/- 28 vs. 58 +/- 11 min, respectively, P < 0.05). FAT increased the activities of the enzymes citrate synthase, beta-hydroxyacyl-CoA dehydrogenase and carnitine palmitoyl-transferase compared to CHO (P < 0.01), but there was no systematic effect of training. We conclude: (1) there was no additive effect of a high-fat diet on endurance performance when rats performed low-intensity training; (2) running performance at 28 m min(-1) was only enhanced by a high-fat diet after more intense training; (3) diet-induced and training-induced adaptations that increase exercise capacity may be under independent control. Experimental Physiology (2001) 86.4, 499-508.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-Hydroxyacyl CoA Dehydrogenases / metabolism
  • Animals
  • Blood Glucose
  • Body Mass Index
  • Carnitine O-Palmitoyltransferase / metabolism
  • Citrate (si)-Synthase / metabolism
  • Diet
  • Dietary Carbohydrates / pharmacology*
  • Dietary Fats / pharmacology*
  • Drinking / physiology
  • Energy Metabolism / physiology
  • Female
  • Glycogen / metabolism
  • Liver / metabolism
  • Muscle, Skeletal / enzymology
  • Physical Conditioning, Animal / physiology*
  • Physical Endurance / physiology*
  • Rats
  • Rats, Sprague-Dawley


  • Blood Glucose
  • Dietary Carbohydrates
  • Dietary Fats
  • Glycogen
  • 3-Hydroxyacyl CoA Dehydrogenases
  • Carnitine O-Palmitoyltransferase
  • Citrate (si)-Synthase