Changes in sodium or glucose filtration rate modulate expression of glucose transporters in renal proximal tubular cells of rat

J Membr Biol. 2001 Jul 15;182(2):105-12. doi: 10.1007/s00232-001-0036-y.


Renal glucose reabsorption is mediated by luminal sodium-glucose cotransporters (SGLTs) and basolateral facilitative glucose transporters (GLUTs). The modulators of these transporters are not known, and their substrates glucose and Na+ are potential candidates. In this study we examined the role of glucose and Na+ filtration rate on gene expression of glucose transporters in renal proximal tubule. SGLT1, SGLT2, GLUT1 and GLUT2 mRNAs were assessed by Northern blotting; and GLUT1 and GLUT2 proteins were assessed by Western blotting. Renal cortex and medulla samples from control rats (C), diabetic rats (D) with glycosuria, and insulin-resistant 15-month old rats (I) without glycosuria; and from normal (NS), low (LS), and high (HS) Na+-diet fed rats were studied. Compared to C and I rats, D rats increased (P < 0.05) gene expression of SGLT2 by approximately 36%, SGLT1 by approximately 20%, and GLUT2 by approximately 100%, and reduced (P < 0.05) gene expression of GLUT1 by more than 50%. Compared to NS rats, HS rats increased (P < 0.05) SGLT2, GLUT2, and GLUT1 expression by approximately 100%, with no change in SGLT1 mRNA expression, and LS rats increased (P < 0.05) GLUT1 gene expression by approximately 150%, with no changes in other transporters. In summary, the results showed that changes in glucose or Na+ filtrated rate modulate the glucose transporters gene expression in epithelial cells of the renal proximal tubule.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Diabetes Mellitus, Experimental / genetics
  • Diabetes Mellitus, Experimental / metabolism
  • Diet
  • Gene Expression Regulation*
  • Glomerular Filtration Rate
  • Glucose / metabolism*
  • Glucose Transporter Type 1
  • Glucose Transporter Type 2
  • Kidney Tubules, Proximal / metabolism*
  • Male
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Monosaccharide Transport Proteins / genetics*
  • Monosaccharide Transport Proteins / metabolism
  • RNA, Messenger
  • Rats
  • Rats, Wistar
  • Sodium / metabolism*
  • Sodium-Glucose Transporter 1
  • Sodium-Glucose Transporter 2


  • Glucose Transporter Type 1
  • Glucose Transporter Type 2
  • Membrane Glycoproteins
  • Monosaccharide Transport Proteins
  • RNA, Messenger
  • Slc2a1 protein, rat
  • Slc5a1 protein, rat
  • Slc5a2 protein, rat
  • Sodium-Glucose Transporter 1
  • Sodium-Glucose Transporter 2
  • Sodium
  • Glucose