Identification and Characterization of the UL24 Gene Product of Herpes Simplex Virus Type 2

Virus Genes. 2001 Jun;22(3):321-7. doi: 10.1023/a:1011118424474.

Abstract

The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • COS Cells
  • Capsid / metabolism
  • DNA Primers
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique, Indirect
  • Herpesvirus 2, Human / metabolism*
  • Immune Sera
  • Viral Proteins / genetics
  • Viral Proteins / immunology
  • Viral Proteins / metabolism*
  • Virion / metabolism

Substances

  • DNA Primers
  • Immune Sera
  • Viral Proteins