Identification of a plasmid encoding SHV-12, TEM-1, and a variant of IMP-2 metallo-beta-lactamase, IMP-8, from a clinical isolate of Klebsiella pneumoniae

Antimicrob Agents Chemother. 2001 Aug;45(8):2368-71. doi: 10.1128/AAC.45.8.2368-2371.2001.

Abstract

A multidrug-resistant plasmid encoding TEM-1, SHV-12, and a variant of IMP-2 metallo-beta-lactamase, designated IMP-8, was identified from a clinical isolate of Klebsiella pneumoniae. There are four nucleotide differences between bla(IMP-2) and bla(IMP-8), resulting in two amino acid differences. bla(IMP-8) was also found to be carried by an integron-borne gene cassette similar to the bla(IMP-2) cassette.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins*
  • Base Sequence
  • Cloning, Molecular
  • Drug Resistance, Microbial / genetics
  • Drug Resistance, Multiple
  • Humans
  • Isoelectric Focusing
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / isolation & purification
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Plasmids / genetics*
  • beta-Lactamases / genetics*
  • beta-Lactamases / isolation & purification
  • beta-Lactamases / metabolism

Substances

  • Bacterial Proteins
  • IMP-8 enzyme
  • beta-lactamase SHV-12
  • beta-Lactamases
  • beta-lactamase TEM-1

Associated data

  • GENBANK/AF322577