The IsdA gene is necessary for sexual development inhibition by a salt in Aspergillus nidulans

Curr Genet. 2001 Jun;39(4):237-43. doi: 10.1007/s002940100206.


Using one of 17 expressed sequence tags (ESTs) previously identified as specific to the late sexual developmental (LSD) stage of Aspergillus nidulans, a gene for the subject EST was isolated. The determined DNA sequences revealed an open reading frame encoding a 356 amino acid polypeptide which does not share a sequence similarity to previously identified genes or proteins. The isolated gene was named lsdA (late sexual development), since it was expressed abundantly at the LSD stage. The lsdA gene expression began earlier than at the LSD stage. Disruption of the lsdA gene in the veA+ background strongly induced sexual development under conditions where sexual development in wild-type strains is almost completely inhibited. In contrast, in the veA1 background, an lsdA null mutant failed to show any phenotypic difference in sexual development under the various conditions tested. These results indicate that the lsdA gene may be responsible for inhibiting the sexual development of veA+ strains by a high concentration of a salt.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Formation
  • Aspergillus nidulans / drug effects
  • Aspergillus nidulans / growth & development
  • Aspergillus nidulans / physiology*
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / chemistry
  • DNA, Fungal / metabolism
  • Expressed Sequence Tags
  • Female
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Dosage
  • Gene Expression Regulation, Fungal / drug effects*
  • Gene Expression Regulation, Fungal / physiology
  • Immunoassay
  • Magnesium Chloride / pharmacology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Mutation
  • Mycotoxins*
  • Oxidoreductases*
  • Phenotype
  • Polymerase Chain Reaction
  • Potassium Chloride / pharmacology*
  • Sodium Chloride / pharmacology*


  • DNA Primers
  • DNA, Fungal
  • Fungal Proteins
  • LSDA protein, Emericella nidulans
  • Mycotoxins
  • Magnesium Chloride
  • Sodium Chloride
  • Potassium Chloride
  • Oxidoreductases
  • STCU protein, Emericella nidulans