Multilayer amniotic membrane transplantation in severe ocular graft versus host disease

Eur J Ophthalmol. Apr-Jun 2001;11(2):183-6. doi: 10.1177/112067210101100215.


Purpose: To clarify the usefulness of multilayer amniotic membrane transplantation in an unusual case of calcareous corneal degeneration in a patient with graft-versus-host disease.

Methods: A 20-year-old Caucasian woman had bilateral calcareous corneal degeneration of one year of evolution, secondary to graft-versus-host disease. Treatment for both eyes with topical steroids and antibiotic ointment was not successful. Right eye had a spontaneous corneal perforation, and a three-layer circle amniotic membrane graft was applied to the whole cornea.

Results: During a follow-up period of 20 months we observed stability of the corneal epithelium and stroma. The amniotic membrane-covered area showed rapid epithelization, reduced inflammation and suppressed fibrosis formation.

Conclusions: Multilayer amniotic membrane transplantation may be considered an alterantive for reconstructing the ocular surface in a patient with severe dry eyes and calcareous corneal degeneration, even with little perforation.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Amnion / transplantation*
  • Corneal Diseases / pathology
  • Corneal Diseases / surgery*
  • Corneal Stroma / pathology
  • Epithelium, Corneal / pathology
  • Female
  • Follow-Up Studies
  • Graft vs Host Disease / pathology
  • Graft vs Host Disease / surgery*
  • Humans
  • Rupture, Spontaneous
  • Visual Acuity