Protective CTL response is induced in the absence of CD4+ T cells and IFN-gamma by gene gun DNA vaccination with a minigene encoding a CTL epitope of Listeria monocytogenes

Vaccine. 2001 Jul 20;19(30):4297-306. doi: 10.1016/s0264-410x(01)00146-3.

Abstract

Our work was undertaken to learn the mechanism of induction of protective cytotoxic T lymphocytes (CTL) by gene gun DNA vaccination with p91m encoding an H-2Kd-restricted T cell epitope of listeriolysin O (LLO). Vaccination with p91m induced vigorous antigen-specific CD8+ CTL that produce IFN-gamma and was able to confer partial protection against listerial challenge. However, the p91m-induced protective immunity was revealed to be independent of the IFN-gamma and CD4+ T cell help. The CTL induction is also suggested to require neither adjuvant activity of the plasmid used nor IFN-gamma. The data may be feasible for the design of CTL inducing vaccines in various immunodeficiencies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Toxins*
  • Bacterial Vaccines / immunology*
  • Biolistics
  • CD4-Positive T-Lymphocytes / physiology*
  • Epitopes, T-Lymphocyte*
  • H-2 Antigens / physiology*
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / immunology*
  • Hemolysin Proteins
  • Interferon-gamma / physiology*
  • Listeria monocytogenes / immunology*
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocytes, Cytotoxic / immunology*
  • Vaccination
  • Vaccines, DNA / immunology*

Substances

  • Bacterial Toxins
  • Bacterial Vaccines
  • Epitopes, T-Lymphocyte
  • H-2 Antigens
  • H-2K(K) antigen
  • Heat-Shock Proteins
  • Hemolysin Proteins
  • Vaccines, DNA
  • Interferon-gamma
  • hlyA protein, Listeria monocytogenes