Production of multiple plant hormones from a single polyprotein precursor

Nature. 2001 Jun 14;411(6839):817-20. doi: 10.1038/35081107.

Abstract

Some animal and yeast hormone genes produce prohormone polypeptides that are proteolytically processed to produce multiple copies of hormones with the same or different functions. In plants, four polypeptides have been identified that can be classed as hormones (intercellular chemical messengers) but none are known to be produced as multiple copies from a single precursor. Here we describe a polyprotein hormone precursor, present in tobacco plants, that gives rise to two polypeptide hormones, as often found in animals and yeast. The tobacco polypeptides activate the synthesis of defensive proteinase-inhibitor proteins in a manner similar to that of systemin, an 18-amino-acid polypeptide found in tomato plants. The two tobacco polypeptides are derived from each end of a 165-amino-acid precursor that bears no homology to tomato prosystemin. The data show that structurally diverse polypeptide hormones in different plant species can serve similar signalling roles, a condition not found in animals or yeast.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cells, Cultured
  • Molecular Sequence Data
  • Peptides / chemistry
  • Peptides / metabolism
  • Plant Growth Regulators / biosynthesis*
  • Plant Proteins / chemistry
  • Plant Proteins / metabolism
  • Plants, Toxic*
  • Polyproteins / metabolism*
  • Protease Inhibitors / metabolism
  • Protein Precursors / metabolism*
  • Tobacco / metabolism*

Substances

  • Peptides
  • Plant Growth Regulators
  • Plant Proteins
  • Polyproteins
  • Protease Inhibitors
  • Protein Precursors
  • systemin

Associated data

  • GENBANK/AY033148
  • GENBANK/AY033149