The C. elegans E2F- and DP-related proteins are required for embryonic asymmetry and negatively regulate Ras/MAPK signaling

Mol Cell. 2001 Mar;7(3):451-60. doi: 10.1016/s1097-2765(01)00193-9.

Abstract

Early C. elegans embryos exhibit protein asymmetries that allow rapid diversification of cells. Establishing these asymmetries requires the novel protein MEX-5. We show that mutations in the efl-1 and dpl-1 genes cause defects in protein localization resembling defects caused by mutations in mex-5. efl-1 and dpl-1 encode homologs of vertebrate E2F and DP proteins that regulate transcription as a heterodimer. efl-1 and dpl-1 mutants have elevated levels of activated Map kinase in oocytes. Their mutant phenotype and that of mex-5 mutants can be suppressed by reducing Ras/Map kinase signaling. We propose this signaling pathway has a role in embryonic asymmetry and that EFL-1/DPL-1 control the level of Map kinase activation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Body Patterning* / genetics
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / embryology*
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins*
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Cell Cycle Proteins*
  • Cell Division
  • DNA-Binding Proteins*
  • Dimerization
  • E2F Transcription Factors
  • Enzyme Activation
  • Female
  • Fertilization / genetics
  • Genes, Helminth / genetics
  • Helminth Proteins / genetics
  • Helminth Proteins / metabolism*
  • MAP Kinase Signaling System
  • Mitogen-Activated Protein Kinases / antagonists & inhibitors*
  • Mitogen-Activated Protein Kinases / metabolism
  • Mosaicism / genetics
  • Mutation / genetics
  • Ovulation / genetics
  • Ovum / metabolism
  • Phenotype
  • Protein Transport
  • Signal Transduction*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • ras Proteins / antagonists & inhibitors*
  • ras Proteins / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • Helminth Proteins
  • MEX-5 protein, C elegans
  • Transcription Factors
  • efl-1 protein, C elegans
  • skn-1 protein, C elegans
  • Mitogen-Activated Protein Kinases
  • ras Proteins