A transient histone hyperacetylation signal marks nucleosomes for remodeling at the PHO8 promoter in vivo

Mol Cell. 2001 Mar;7(3):529-38. doi: 10.1016/s1097-2765(01)00200-3.

Abstract

Chromatin remodeling of the yeast PHO8 promoter requires the SAGA histone acetyltransferase complex. We report here that SAGA is necessary and sufficient to establish an activator-dependent hyperacetylation peak over the PHO8 promoter that is restricted to those nucleosomes that are remodeled upon activation. This local hyperacetylated state is observed upon activation in the absence of the SWI/SNF complex when the remodeling process is frozen subsequent to activator binding. Hyperacetylation is lost, however, if remodeling is permitted to go to completion. Thus, a transient histone hyperacetylation signal is shown to be a prerequisite for, and determinant of, the domain of nucleosome remodeling in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Acetyltransferases / genetics
  • Acetyltransferases / metabolism*
  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism
  • Chromosomal Proteins, Non-Histone
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • DNA-Binding Proteins*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Fungal
  • Genes, Fungal / genetics*
  • Histone Acetyltransferases
  • Histone Deacetylases
  • Histones / chemistry*
  • Histones / metabolism*
  • Macromolecular Substances
  • Molecular Conformation
  • Nucleosomes / chemistry*
  • Nucleosomes / genetics
  • Nucleosomes / metabolism*
  • Phosphates / pharmacology
  • Precipitin Tests
  • Promoter Regions, Genetic / genetics*
  • Protein Kinases / genetics
  • Protein Kinases / metabolism
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / physiology
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription Factors / physiology
  • Transcriptional Activation

Substances

  • Chromosomal Proteins, Non-Histone
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Histones
  • Macromolecular Substances
  • Nucleosomes
  • PHO4 protein, S cerevisiae
  • Phosphates
  • SWI1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Acetyltransferases
  • Histone Acetyltransferases
  • Protein Kinases
  • SNF1-related protein kinases
  • Protein-Serine-Threonine Kinases
  • RPD3 protein, S cerevisiae
  • Histone Deacetylases
  • Adenosine Triphosphatases