Thioredoxin peroxidase-1 (peroxiredoxin-1) is increased in thioredoxin-1 transfected cells and results in enhanced protection against apoptosis caused by hydrogen peroxide but not by other agents including dexamethasone, etoposide, and doxorubicin

Arch Biochem Biophys. 2001 Aug 1;392(1):103-9. doi: 10.1006/abbi.2001.2435.


Thioredoxin-1 (Trx-1) is a small redox oncoprotein whose expression is increased in a number of human primary cancers where it is associated with aggressive tumor growth, inhibition of apoptosis and decreased patient survival. We report that Trx-1-transfected MCF-7 human breast cancer cells have increased expression of thioredoxin peroxidase-1 (TrxP-1) a peroxiredoxin family member that scavenges H(2)O(2) using Trx-1 as a source of reducing equivalents. Our work shows that TrxP-1 is more effective than selenium-dependent glutathione peroxidase in protecting cells against H(2)O(2) damage. Transfection of mouse WEHI7.2 lymphoma cells with human TrxP-1 or TrxP-2, but not TrxP-4, protects the cells against H(2)O(2) induced apoptosis but does not protect against apoptosis induced by dexamethasone, etoposide, or doxorubicin. The results show that an increase in TrxP-1 expression contributes to the protection against H(2)O(2) induced apoptosis caused by Trx-1, but does not protect against apoptosis induced by other agents.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Apoptosis / drug effects*
  • Apoptosis / genetics
  • Apoptosis / physiology*
  • Base Sequence
  • DNA Primers / genetics
  • Dexamethasone / pharmacology
  • Doxorubicin / pharmacology
  • Etoposide / pharmacology
  • Female
  • Gene Expression
  • Humans
  • Hydrogen Peroxide / pharmacology*
  • Mice
  • Neoplasm Proteins*
  • Peroxidases / genetics
  • Peroxidases / metabolism*
  • Peroxiredoxin III
  • Peroxiredoxins
  • Selenium / pharmacology
  • Thioredoxins / genetics*
  • Thioredoxins / metabolism*
  • Transfection
  • Tumor Cells, Cultured


  • Antioxidants
  • DNA Primers
  • Neoplasm Proteins
  • Prdx3 protein, mouse
  • Thioredoxins
  • Etoposide
  • Dexamethasone
  • Doxorubicin
  • Hydrogen Peroxide
  • Peroxidases
  • PRDX1 protein, human
  • PRDX3 protein, human
  • Peroxiredoxin III
  • Peroxiredoxins
  • Selenium