DNA polymerase beta is not essential for the formation of palindromic (P) region of T cell receptor gene

Immunol Lett. 2001 Aug 1;78(1):45-9. doi: 10.1016/s0165-2478(01)00232-2.


Formation of palindromic (P) region at the variable (V)-diversity (D)-joining (J) junction in DNA polymerase beta (pol-beta) deficient mice were investigated by sequencing of reverse transcriptase-polymerase chain reaction (RT-PCR) products of mRNAs encoding the beta chain of T cell receptor (TCR). Total 42 and 43 cDNA clones encoding V(beta8)-D(beta)-J(beta)-C(beta) from E18.5 embryonic thymocytes of pol-beta gene knocked-out and wild type control mouse, respectively, were sequenced. Among them five and six clones from pol-beta knocked-out and wild type, respectively, have P insertions of two nucleotides. This result unequivocally indicates that pol-beta, which is one of the repair-type DNA polymerases most abundantly expressed in thymus and spleen, is not essential for the formation of P region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Polymerase beta / deficiency*
  • DNA Polymerase beta / genetics
  • Immunoglobulin Joining Region / genetics
  • Immunoglobulin Variable Region / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • RNA, Messenger / genetics
  • Receptors, Antigen, T-Cell / genetics*


  • Immunoglobulin Joining Region
  • Immunoglobulin Variable Region
  • RNA, Messenger
  • Receptors, Antigen, T-Cell
  • DNA Polymerase beta