Molecular cloning and characterization of a novel SH3 protein (SLY) preferentially expressed in lymphoid cells

Biochim Biophys Acta. 2001 Jul 30;1520(1):89-93. doi: 10.1016/s0167-4781(01)00242-1.


A novel full-length cDNA was isolated from a murine T-cell lymphoma library that has an open reading frame encoding 381 amino acids. The predicted protein (termed SLY) contains a Src homology 3 domain and a sterile alpha motif, suggesting that it functions as a signaling adaptor protein in lymphocytes. Northern blot and in situ hybridization analysis showed a preferential expression in lymphoid tissues. The sly gene is located on the X-chromosome in close proximity to genes involved in various immune disorders. This is consistent with an additional role of SLY in immune pathology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / isolation & purification
  • Gene Library
  • Immediate-Early Proteins*
  • In Situ Hybridization
  • Lymphoid Tissue / metabolism
  • Lymphoma, T-Cell / genetics
  • Lymphoma, T-Cell / metabolism
  • Mice
  • Munc18 Proteins
  • RNA, Messenger / biosynthesis
  • Signal Transduction
  • Transfection
  • src Homology Domains*


  • Carrier Proteins
  • DNA, Complementary
  • Immediate-Early Proteins
  • Munc18 Proteins
  • RNA, Messenger
  • Scfd1 protein, rat