An Alu cassette in the human epithelial sodium channel

Biochim Biophys Acta. 2001 Jul 30;1520(1):94-8. doi: 10.1016/s0167-4781(01)00244-5.

Abstract

Here, we report the presence of two splice variants of the human epithelial sodium channel alpha subunit (h alpha ENaC) containing Alu cassette, namely h alpha ENaC+22 and h alpha ENaC+Alu, in various tissues. Functional expression of these splice variants with hENaC beta and gamma subunits produced loss-of-channel activity in the Xenopus oocyte expression system. Interestingly, coexpression of h alpha ENaC+22 or h alpha ENaC+Alu, respectively, with wild type hENaC alpha, beta, and gamma subunits enhanced the expression of amiloride-sensitive current in oocytes. The presence of Alu sequences in the 3'-untranslated region of h gamma ENaC was also identified.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Alu Elements*
  • Amino Acid Sequence
  • Base Sequence
  • Epithelium / metabolism*
  • Humans
  • Models, Chemical
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sodium Channels / metabolism*

Substances

  • Sodium Channels

Associated data

  • GENBANK/AF087510
  • GENBANK/AF087511