Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1

FEMS Microbiol Lett. 2001 Jul 24;201(2):237-41. doi: 10.1111/j.1574-6968.2001.tb10762.x.


Six non-clonally related enterobacterial isolates producing a same extended-spectrum beta-lactamase CTX-M-15 were isolated in 1999 from patients hospitalized in a New Delhi hospital. CTX-M-15 differed from CTX-M-3 by an asparagine to glycine substitution in position ABL238. Its gene was located on large plasmids varying in size. In each case, a same insertion sequence ISEcp1 was identified upstream of the 5' end of bla(CTX-M-15). Typical -35 and -10 promoter sequences of Enterobacteriaceae were identified in the 3' end of ISEcp1. The location of ISEcp1 upstream of plasmid-mediated CTX-M-type beta-lactamase genes may contribute to their spread or/and their expression.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Transposable Elements / genetics*
  • Drug Resistance, Bacterial / genetics*
  • Enterobacteriaceae / drug effects
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / genetics*
  • Enterobacteriaceae Infections / epidemiology
  • Enterobacteriaceae Infections / microbiology
  • Genes, Bacterial / genetics
  • Humans
  • India / epidemiology
  • Isoelectric Focusing
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Polymerase Chain Reaction
  • Random Amplified Polymorphic DNA Technique
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism*


  • DNA Transposable Elements
  • beta-Lactamases