Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression

Comp Biochem Physiol B Biochem Mol Biol. 2001 Aug;130(1):13-21. doi: 10.1016/s1096-4959(01)00381-5.

Abstract

In the present study, the Sparus aurata alpha-skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream alpha-actin is identical to Fugu alpha-actin-1. The expression of alpha-actin was initiated at the onset of segmentation. In adult fish, alpha-actin is expressed predominantly in white and red muscle.

MeSH terms

  • Actins / biosynthesis*
  • Actins / genetics*
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Fishes
  • Gene Library
  • Molecular Sequence Data
  • Muscles / metabolism
  • Phylogeny
  • RNA / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sea Bream
  • Sequence Homology, Amino Acid
  • Time Factors
  • Tissue Distribution

Substances

  • Actins
  • DNA, Complementary
  • RNA

Associated data

  • GENBANK/AF190473