Acetate, propionate and butyrate in plasma: determination of the concentration and isotopic enrichment by gas chromatography/mass spectrometry with positive chemical ionization

J Mass Spectrom. 2001 Jul;36(7):798-805. doi: 10.1002/jms.181.

Abstract

This study describes a rapid and simple method to determine short-chain fatty acid (SCFA) concentrations and their isotopic enrichments (M(0) + 1 and M(0) + 2) in human plasma. Sample preparation involves SCFA extraction and derivatization with 1-(tert-butyldimethylsilyl)imidazole. Gas chromatography/mass spectrometry was performed using chemical ionization with ammonia as the reagent gas. Outstanding resolution, excellent linearity and good detection limits were obtained. Inter-assay and intra-assay repeatability was below 10% and 3% respectively for SCFA concentration. Inter-assay repeatability was below 5%, 4%, 6%, and 14% for isotopic enrichment determination of [1-(13)C]acetate and [1,2-(13)C(2)]acetate, [1-(13)C]propionate and [1-(13)C]butyrate respectively, with intra-assay being below 6%. Such SCFA concentrations and isotopic enrichments were determined in the plasma of rats infused with a (13)C-labeled SCFA. The turnovers of acetate, propionate and butyrate in rats were 19 micromol kg(-1) min(-1), 2.6 micromol kg(-1) min(-1), 0.3 micromol kg(-1) min(-1) respectively.

MeSH terms

  • Acetates / administration & dosage
  • Acetates / blood*
  • Acetates / pharmacokinetics
  • Animals
  • Butyrates / administration & dosage
  • Butyrates / blood*
  • Butyrates / pharmacokinetics
  • Carbon Isotopes
  • Fasting
  • Fatty Acids, Volatile / blood
  • Gas Chromatography-Mass Spectrometry / methods*
  • Humans
  • Propionates / administration & dosage
  • Propionates / blood*
  • Propionates / pharmacokinetics
  • Rats
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Acetates
  • Butyrates
  • Carbon Isotopes
  • Fatty Acids, Volatile
  • Propionates
  • propionic acid