In vivo inhibition of renal 11beta-hydroxysteroid dehydrogenase in the rat stimulates collecting duct sodium reabsorption

Clin Sci (Lond). 2001 Aug;101(2):195-8.


In order to test the proposal that the aldosterone specificity of mineralocorticoid receptors in the collecting duct depends on inactivation of glucocorticoids by the enzyme 11beta-hydroxysteroid dehydrogenase (11beta-HSD), we have assessed the effect of pharmacological inhibition of 11beta-HSD on collecting duct Na+ reabsorption in vivo. Adrenalectomized rats (n=14) were infused intravenously with high-dose corticosterone, and late-distal tubules were perfused orthogradely with artificial tubular fluid containing [14C]inulin and 22Na; urinary recoveries of the radioisotopes were monitored. Half of the rats received intravenous carbenoxolone to inhibit renal 11beta-HSD activity. The urinary recovery of [14C]inulin was complete in both groups of animals (101+/-2% versus 101+/-3%), but the recovery of 22Na was lower in carbenoxolone-treated rats (34+/-5%) than in the corticosterone-alone group (54+/-4%, P<0.01). These data, which provide the first demonstration of enhanced Na+ reabsorption in the distal nephron during inhibition of renal 11beta-HSD in vivo, strongly support the proposal that 11beta-HSD normally prevents endogenous glucocorticoid from exerting mineralocorticoid-like effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorption
  • Adrenalectomy
  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Carbenoxolone / pharmacology
  • Carbon Radioisotopes / metabolism
  • Corticosterone / administration & dosage
  • Enzyme Inhibitors / pharmacology
  • Hydroxysteroid Dehydrogenases / antagonists & inhibitors
  • Hydroxysteroid Dehydrogenases / physiology*
  • Kidney Tubules, Collecting / metabolism*
  • Photometry
  • Rats
  • Rats, Sprague-Dawley
  • Sodium / pharmacokinetics*
  • Sodium Radioisotopes / metabolism


  • Anti-Inflammatory Agents
  • Carbon Radioisotopes
  • Enzyme Inhibitors
  • Sodium Radioisotopes
  • Sodium
  • Hydroxysteroid Dehydrogenases
  • Carbenoxolone
  • Corticosterone