A wireless electrochemiluminescence detector applied to direct and indirect detection for electrophoresis on a microfabricated glass device

A Arora; J C Eijkel; W E Morf; A Manz

doi:10.1021/ac0100300

A wireless electrochemiluminescence detector applied to direct and indirect detection for electrophoresis on a microfabricated glass device

Anal Chem. 2001 Jul 15;73(14):3282-8. doi: 10.1021/ac0100300.

Authors

A Arora¹, J C Eijkel, W E Morf, A Manz

Affiliation

¹ Astra Zeneca/SmithKline Beecham Centre for Analytical Sciences, Department of Chemistry, Imperial College of Science, Technology & Medicine, South Kensington, London, United Kingdom.

PMID: 11476226
DOI: 10.1021/ac0100300

Abstract

A novel electrochemiluminescence (ECL) detector is presented in this article. The detector is applied for micellar electrokinetic chromatographic separation of dichlorotris(2,2'-bipyridyl)ruthenium(II) hydrate [Ru-(bpy)] and dichlorotris(1,10-phenanthroline)ruthenium-(II) hydrate [Ru(phen)] on a microfabricated glass device. It consists of a microfabricated "U"-shape floating platinum electrode placed across the separation channel. The legs of the U function respectively as working and counter electrode. The required potential difference for the ECL reaction is generated at the Pt electrode by the electric field available in the separation channel during electrophoretic separation. Initial experiments demonstrate a micellar electrokinetic separation and direct ECL detection of 10(-16) mol of Ru(phen) (10(-6) M) and 4.5 x 10(-16) mol of Ru(bpy) (5 x 10(-6) M). Also, preliminary results show the indirect detection of three amino acids. The high voltage at the location of detection does not interfere with the electrochemistry.