Abstract
A new antibiotic designated TMC-69 has been isolated from the fermentation broth of a fungal strain Chrysosporium sp. TC 1068. TMC-69 exhibited moderate in vitro cytotoxic activity. TMC-69-6H, a derivative of TMC-69 prepared by hydrogenation, possessed more potent in vitro cytotoxicity than TMC-69, and exhibited in vivo antitumor activity against murine P388 leukemia and B16 melanoma. TMC-69-6H was found to specifically inhibit Cdc25A and B phosphatases.
MeSH terms
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Animals
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Antibiotics, Antineoplastic / pharmacology*
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Biological Assay
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Cell Division / drug effects
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Chrysosporium / growth & development
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Chrysosporium / metabolism*
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Enzyme Inhibitors / pharmacology*
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Humans
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Hydrogenation
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Inhibitory Concentration 50
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Leukemia P388 / drug therapy
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Melanoma, Experimental / drug therapy
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Mice
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Pyrans / pharmacology*
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Tumor Cells, Cultured
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cdc25 Phosphatases / antagonists & inhibitors*
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cdc25 Phosphatases / metabolism
Substances
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Antibiotics, Antineoplastic
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Enzyme Inhibitors
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Pyrans
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TMC-69
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TMC-69-6H
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CDC25A protein, human
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Cdc25a protein, mouse
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cdc25 Phosphatases