Identification of a new imprinted gene, Rian, on mouse chromosome 12 by fluorescent differential display screening

I Hatada; S Morita; Y Obata; Y Sotomaru; M Shimoda; T Kono

doi:10.1093/oxfordjournals.jbchem.a002971

Identification of a new imprinted gene, Rian, on mouse chromosome 12 by fluorescent differential display screening

J Biochem. 2001 Aug;130(2):187-90. doi: 10.1093/oxfordjournals.jbchem.a002971.

Authors

I Hatada¹, S Morita, Y Obata, Y Sotomaru, M Shimoda, T Kono

Affiliation

¹ Gene Research Center, Gunma University, Showa-machi, Maebashi, Gunma 371-8511, Japan. ihatada@showa.gunma-u.ac.jp

PMID: 11481034
DOI: 10.1093/oxfordjournals.jbchem.a002971

Abstract

Systematic screening of differentially expressed genes among androgenetic, parthenogenetic, and normal embryos by means of fluorescent differential display revealed five imprinted genes. One of them, named Rian, was expressed exclusively from the maternal allele and was closely linked to an imprinted gene, Meg3(Gtl2), mapped to the distal end of chromosome 12. The Rian transcript did not have any apparent open reading frame, and its transcript was exclusively localized to the nucleus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Embryo, Mammalian / metabolism*
Gene Expression Profiling / methods*
Genes
Genomic Imprinting*
In Situ Hybridization, Fluorescence
Mice
Molecular Sequence Data
Nuclear Proteins / genetics*
Open Reading Frames
RNA, Messenger / genetics
RNA, Messenger / metabolism
Radiation Hybrid Mapping

Substances

Nuclear Proteins
RNA, Messenger
Rian protein, mouse