Monitoring actin folding. Purification protocols for labeled proteins and binding to DNase I-sepharose beads

Methods Mol Biol. 2000:140:161-7. doi: 10.1385/1-59259-061-6:161.

Authors

V Thulasiraman¹, R G Ferreyra, J Frydman

Affiliation

¹ Department of Biological Sciences, Stanford University, Stanford, CA, USA.

PMID: 11484485
DOI: 10.1385/1-59259-061-6:161

No abstract available

MeSH terms

Actins / chemistry*
Actins / isolation & purification
Actins / metabolism*
Animals
CHO Cells
Chickens
Chromatography, Affinity / methods
Cloning, Molecular / methods
Cricetinae
Deoxyribonuclease I
Guanidine
Indicators and Reagents
Protein Denaturation
Protein Folding*
Recombinant Proteins / chemistry
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Sepharose
Sulfur Radioisotopes
Transfection / methods

Substances

Actins
Indicators and Reagents
Recombinant Proteins
Sulfur Radioisotopes
Sepharose
Deoxyribonuclease I
Guanidine