Infectious DNA clone of HIV type 1 A/G recombinant (CRF02_AG) replicable in peripheral blood mononuclear cells

AIDS Res Hum Retroviruses. 2001 Jul 20;17(11):1083-7. doi: 10.1089/088922201300343771.

Abstract

We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02_AG) and was found to contain the least amount of subtype G-derived region among the known CRF02_AG HIV-1 DNAs. This result suggests that CRF02_AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGIC5, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02_AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Cloning, Molecular
  • DNA, Recombinant / genetics
  • DNA, Recombinant / isolation & purification
  • HIV Infections / genetics*
  • HIV Infections / transmission
  • HIV Infections / virology
  • HIV-1 / genetics*
  • Humans
  • Leukocytes, Mononuclear / virology
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Analysis, DNA
  • Transfection
  • Tumor Cells, Cultured
  • Virus Replication

Substances

  • DNA, Recombinant

Associated data

  • GENBANK/AB052867