B-myb rescues ras-induced premature senescence, which requires its transactivation domain

Cancer Lett. 2001 Sep 28;171(1):87-101. doi: 10.1016/s0304-3835(01)00631-0.

Abstract

B-myb, a ubiquitously expressed member of the myb gene family, is highly regulated throughout the cell cycle and appears to be required for cell cycle progression. In contrast to its relatives A-myb, c-myb, and v-myb, no transforming activity of B-myb has been reported thus far. We report here that B-myb can rescue senescence induced by an activated ras oncogene in rodent cells in vitro. We show that transformation by B-Myb involves its ability to activate transcription. Similar to other oncogenic transcription factors, such as c-Myc and E2F, we show that B-Myb also has repression activity. We demonstrate that the C-terminus of B-Myb can function as a repressor of transcription, that B-Myb interacts with the repressor molecules BS69 and N-CoR and that the repression function, like the transactivation domain, contributes to B-myb transformation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Carrier Proteins / physiology
  • Cell Cycle Proteins*
  • Cellular Senescence / physiology*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / physiology*
  • Gene Expression Regulation*
  • Humans
  • Mice
  • Nuclear Proteins / metabolism
  • Nuclear Proteins / physiology
  • Nuclear Receptor Co-Repressor 1
  • Protein Structure, Tertiary
  • Proteins / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / physiology*
  • Retinoblastoma-Like Protein p107
  • Trans-Activators / chemistry
  • Trans-Activators / physiology*
  • Transcriptional Activation*
  • Transfection
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p14ARF
  • Tumor Suppressor Protein p53 / metabolism
  • ras Proteins / physiology*

Substances

  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • MYBL2 protein, human
  • Mybl2 protein, mouse
  • NCOR1 protein, human
  • Ncor1 protein, mouse
  • Nuclear Proteins
  • Nuclear Receptor Co-Repressor 1
  • Proteins
  • Rbl1 protein, mouse
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Retinoblastoma-Like Protein p107
  • Trans-Activators
  • Tumor Suppressor Protein p14ARF
  • Tumor Suppressor Protein p53
  • ZMYND11 protein, human
  • Zmynd11 protein, mouse
  • ras Proteins