Chiral separation of verapamil and some of its metabolites by HPLC and CE

Pharmazie. 2001 Jul;56(7):536-41.

Abstract

HPLC and CE assays were developed for chiral separations of verapamil and its metabolites in serum samples. Three chiral HPLC columns (Chiralcel OJ, Chiralpak AD and Chiralcel OD-R) were tested in normal and reverse-phase modes. All HPLC analyses were performed with fluorescence detection at 276 and 310 nm. CE was realized using CM-beta-CD as a chiral selector for the enantiomeric analysis. The results of HPLC and CE studies were compared and the possibilities for the applications in therapeutic drug monitoring were discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amylose / chemistry
  • Biotransformation
  • Calcium Channel Blockers / chemistry
  • Calcium Channel Blockers / isolation & purification*
  • Calcium Channel Blockers / pharmacokinetics
  • Chromatography, High Pressure Liquid
  • Cyclodextrins
  • Electrophoresis, Capillary
  • Humans
  • Indicators and Reagents
  • Stereoisomerism
  • Verapamil / analogs & derivatives*
  • Verapamil / blood
  • Verapamil / chemistry
  • Verapamil / isolation & purification*
  • Verapamil / pharmacokinetics

Substances

  • Calcium Channel Blockers
  • Cyclodextrins
  • Indicators and Reagents
  • Amylose
  • norverapamil
  • Verapamil