Abstract
HPLC and CE assays were developed for chiral separations of verapamil and its metabolites in serum samples. Three chiral HPLC columns (Chiralcel OJ, Chiralpak AD and Chiralcel OD-R) were tested in normal and reverse-phase modes. All HPLC analyses were performed with fluorescence detection at 276 and 310 nm. CE was realized using CM-beta-CD as a chiral selector for the enantiomeric analysis. The results of HPLC and CE studies were compared and the possibilities for the applications in therapeutic drug monitoring were discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amylose / chemistry
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Biotransformation
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Calcium Channel Blockers / chemistry
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Calcium Channel Blockers / isolation & purification*
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Calcium Channel Blockers / pharmacokinetics
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Chromatography, High Pressure Liquid
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Cyclodextrins
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Electrophoresis, Capillary
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Humans
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Indicators and Reagents
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Stereoisomerism
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Verapamil / analogs & derivatives*
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Verapamil / blood
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Verapamil / chemistry
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Verapamil / isolation & purification*
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Verapamil / pharmacokinetics
Substances
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Calcium Channel Blockers
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Cyclodextrins
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Indicators and Reagents
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Amylose
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norverapamil
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Verapamil