Production of Withaferin A in Shoot Cultures of Withania Somnifera

Planta Med. 2001 Jul;67(5):432-6. doi: 10.1055/s-2001-15811.

Abstract

Multiple shoot cultures of Withania somnifera were established from single shoot tip explants and their potential for the production of two principle withanolides, withaferin A and withanolide D was investigated. Shoot tips grown on MS medium supplemented with BA (1 mg l(-1)) induced 10.0 +/- 1.15 microshoots per explants and shoot cultures accumulated both withanolides (withaferin A = 0.04%, withanolide D = 0.06%). Supplementation of MSSM (solid) agar medium with 4% sucrose enhanced accumulation of both withaferin A (0.16%) and withanolide D (0.08%). Reduction of the agar concentration to 0.16% increased the number of microshoots induced per explant to 25.5. MSSM liquid medium containing 10% coconut milk favoured a maximum increase in biomass (27 fold); number of microshoots induced (37.6 +/- 1.45) as well as accumulation of withaferin A (0.14%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine / analogs & derivatives*
  • Adenine / pharmacology
  • Agar
  • Antineoplastic Agents, Phytogenic / biosynthesis*
  • Benzyl Compounds
  • Carbon / metabolism
  • Culture Media
  • Culture Techniques
  • Ergosterol / analogs & derivatives*
  • Ergosterol / biosynthesis*
  • Kinetin
  • Plant Extracts
  • Plant Growth Regulators / pharmacology
  • Plant Shoots / drug effects
  • Plant Shoots / growth & development
  • Plant Shoots / metabolism*
  • Purines
  • Solanaceae / metabolism*
  • Withanolides

Substances

  • Antineoplastic Agents, Phytogenic
  • Benzyl Compounds
  • Culture Media
  • Plant Extracts
  • Plant Growth Regulators
  • Purines
  • Withanolides
  • withanolide D
  • Carbon
  • Agar
  • Adenine
  • benzylaminopurine
  • withaferin A
  • Kinetin
  • Ergosterol