Determination of the extracellular concentration of glycine in the rat spinal cord dorsal horn by quantitative microdialysis

Brain Res. 2001 Aug 10;910(1-2):192-4. doi: 10.1016/s0006-8993(01)02684-1.


Two quantitative microdialysis methods were used to determine the extracellular concentration of glycine in the dorsal spinal cord of halothane-anaesthetised rats. Extracellular glycine determined by zero net flux was 2.6+/-0.3 microM and by the zero flow method was 3.3+/-0.3 microM. For comparison the glycine content of cerebrospinal fluid was determined to be 6.4+/-1.1 microM. There was no correlation between the extracellular and the cerebrospinal fluid concentrations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Extracellular Space / metabolism*
  • Glycine / cerebrospinal fluid*
  • Male
  • Microdialysis / methods*
  • Posterior Horn Cells / cytology
  • Posterior Horn Cells / metabolism*
  • Rats
  • Rats, Wistar
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Synaptic Transmission / physiology*


  • Receptors, N-Methyl-D-Aspartate
  • Glycine