Further Studies on RpoS in Enterobacteria: Identification of rpoS in Enterobacter Cloacae and Kluyvera Cryocrescens

Arch Microbiol. 2001 Jun;175(6):395-404. doi: 10.1007/s002030100277.

Abstract

RpoS, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. Many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. To determine the presence of this gene in enteric bacteria, an Escherichia coli rpoS probe was constructed and used to detect the presence of this gene in different species. A gene homologous to rpoS was found in Citrobacter amalonaticus, Enterobacter cloacae, Klebsiella planticola, Kluyvera cryocrescens, Serratia rubidaea, Shigella sonnei, and Yersinia ruckeri. Providencia stuartii and Proteus vulgaris were the only tested enterobacteria that did not show any signal with the E. coli rpoS probe or that did not lead to amplification of an rpoS fragment using specific primers. The rpoS gene from E. cloacae and from K. cryocrescens was cloned and sequenced and a mutant allele was constructed in E. cloacae. Survival rates under different harsh conditions were followed in order to determine the effect of rpoS inactivation in exponential- and stationary-phase cells of both strains. E. cloacae rpoS mutants were more sensitive to extreme pH, high osmolarity, and high temperature than the wild-type.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Cloning, Molecular
  • Enterobacter cloacae / chemistry
  • Enterobacter cloacae / cytology
  • Enterobacter cloacae / genetics*
  • Enterobacter cloacae / radiation effects
  • Enterobacteriaceae / chemistry
  • Enterobacteriaceae / classification
  • Enterobacteriaceae / genetics*
  • Enterobacteriaceae / radiation effects
  • Escherichia coli / genetics
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Osmolar Concentration
  • Phylogeny
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sigma Factor / chemistry
  • Sigma Factor / genetics*
  • Sigma Factor / metabolism
  • Temperature
  • Ultraviolet Rays

Substances

  • Bacterial Proteins
  • Sigma Factor
  • sigma factor KatF protein, Bacteria

Associated data

  • GENBANK/AJ002492
  • GENBANK/Y13230