Inhibition of human allergic T-cell responses by IL-10-treated dendritic cells: differences from hydrocortisone-treated dendritic cells

J Allergy Clin Immunol. 2001 Aug;108(2):242-9. doi: 10.1067/mai.2001.117177.


Background: Dendritic cells (DCs) are able to induce human allergic T(H)1 responses as well as T(H)2 responses.

Objective: In this study, we examined the effect of antiinflammatory agents such as IL-10 and hydrocortisone (HC) on the accessory function of DCs and the resulting T-cell response, especially that of T(H)2 cells.

Methods: Naive and memory CD4(+) T cells from atopic donors were stimulated with autologous allergen-pulsed DCs generated from CD14(+) monocytes by culture with GM-CSF/IL-4 and fully matured with IL-1 beta, TNF-alpha, and PGE(2) in the presence or absence of IL-10 or HC.

Results: IL-10-treated DCs and, to a lesser extent, HC-treated DCs showed a decreased expression of MHC II molecules, the costimulatory molecule CD86, and the DC-specific marker CD83, as well as a strongly reduced IL-12 secretion. Consequently, T-cell proliferation was reduced after stimulation with IL-10- or HC-treated DCs alike. However, pretreatment of DCs with IL-10 inhibited the production of T(H)1 and T(H)2 cytokines by T cells, whereas HC-treated DCs inhibited production of IFN-gamma but induced an increased release of IL-4 and no change in IL-5. Both effects were long-lasting; cytokine production remained low (which was due not to enhanced apoptosis but to functional hyporesponsiveness) or even increased after restimulation with fully matured DCs.

Conclusion: These data indicate that IL-10- or HC-treated DCs differ in their ability to influence human allergic T-cell responses. This has major implications for therapeutic strategies aiming at the downregulation of proallergic T(H)2 responses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens
  • Anti-Inflammatory Agents / pharmacology*
  • Antigen Presentation
  • CD4-Positive T-Lymphocytes
  • Cytokines / biosynthesis
  • Dendritic Cells / drug effects*
  • Humans
  • Hydrocortisone / pharmacology*
  • Hypersensitivity, Immediate / immunology*
  • Immunologic Memory
  • Interferon-gamma / biosynthesis
  • Interleukin-10 / pharmacology*
  • Interleukin-4 / biosynthesis
  • Interleukin-5 / biosynthesis
  • Models, Immunological
  • T-Lymphocytes / immunology*
  • Th2 Cells / drug effects


  • Allergens
  • Anti-Inflammatory Agents
  • Cytokines
  • Interleukin-5
  • Interleukin-10
  • Interleukin-4
  • Interferon-gamma
  • Hydrocortisone