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. 2001 Jun;171(5):377-86.
doi: 10.1007/s003600100186.

Inhibitory Non-Genomic Effects of Progesterone on Na+ Absorption in Epithelial Cells From Xenopus Kidney (A6)

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Inhibitory Non-Genomic Effects of Progesterone on Na+ Absorption in Epithelial Cells From Xenopus Kidney (A6)

M Fronius et al. J Comp Physiol B. .

Abstract

The effect of the steroid hormone progesterone on transepithelial sodium transport was measured in confluent monolayers of the A6-cell line from Xenopus kidney. Apical permeabilization with Amphotericin B enabled us to measure the Na+/K+-pump current, and current-fluctuation analysis was used to analyze changes in apical channel density and gating characteristics. Basolateral progesterone (22.2 microM) had a rapid inhibitory effect on the Na+/K+-pump current, and a corresponding decrease in Na+ channel density. The effect occurred within some minutes and took about 50 min to reach a new steady state, in which 45% of the macroscopic current (ISC) was inhibited. Progesterone also inhibits the hypo-osmotic stimulation of Na+ channels which occurs in untreated monolayers. Compared with the known effects of adrenal steroids, our results show a rapid inhibitory action of a steroid hormone on Na+ absorption. The time profile of the progesterone effect suggests, at least in the first minutes, a non-genomic action of progesterone.

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